Covalent modifications of DNA and histones may recruit protein complexes to remodel chromatin, altering its compaction and shaping the three-dimensional topology of the genome. Eukaryotic DNA is wrapped in the nucleus around histone octamers into nucleosomes conforming chromatin structure in association to other proteins and RNA. The IPS2 benefits from the support of the LabExSaclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS).Ĭompeting interests: The authors have declared that no competing interests exist.ĭuring development of multicellular organisms, early embryonic cells must adopt particular gene expression patterns to differentiate into a variety of functionally specialized cells in tissues and organs.
įunding: TJ was Paris-Sud University PhD fellow. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: Data have been deposited to GEO. Received: Accepted: JPublished: July 13, 2016Ĭopyright: © 2016 Veluchamy et al. PLoS ONE 11(7):Įditor: Mohammed Bendahmane, Ecole Normale Superieure, FRANCE (2016) LHP1 Regulates H3K27me3 Spreading and Shapes the Three-Dimensional Conformation of the Arabidopsis Genome. Our work reveals a general role of LHP1 from local to higher conformation levels of chromatin configuration to determine its accessibility to define gene expression patterns.Ĭitation: Veluchamy A, Jégu T, Ariel F, Latrasse D, Mariappan KG, Kim S-K, et al. We also identified a subset of LHP1-activated genes and demonstrated that LHP1 shapes local chromatin topology in order to control transcriptional co-regulation. Here, we show that LHP1 is responsible for the spreading of H3K27me3 towards the 3’ end of the gene body. In Arabidopsis thaliana, LHP1 co-localizes with H3K27me3 epigenetic marks throughout the genome and interacts with PRC1 and PRC2 members as well as with a long noncoding RNA.
Polycomb repressive complexes (PRCs) mediate stable or flexible gene repression in response to internal and environmental cues. Dynamic chromatin conformation and spatial organization of the genome constitute a major step in this regulation to modulate developmental outputs. Precise expression patterns of genes in time and space are essential for proper development of multicellular organisms.
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